Preparation of caffeic acid grafted chitosan self-assembled micelles to enhance oral bioavailability and antibacterial activity of quercetin

Quercetin (QR) is a naturally occurring flavonoid organic compound that has poor solubility in water and highly unstable alkaline conditions, resulting limited absorption poultry. Consequently, our experiment, QR was employed as model compound, encapsulated within the caffeic acid graft chitosan copolymer (CA-g-CS) self-assembled micelles to enhance its solubility, stability exhibit synergistic antibacterial effect. The optimization of formula carried out using combination single-factor experimentation response surface method. vitro release rate CA-g-CS-loaded (CA-g-CS/QR) various pH media were studied pharmacokinetics white feather broiler chickens evaluated vivo . Additionally, activity investigated Escherichia coliCMCC44102 coli chicken origin test strain. results showed optimized for 4 mL water, 0.02 mg/mL copolymer, 1 mg QR, stirring at room temperature. encapsulation efficiency 72.09%. CA-g-CS/QR uniform size with an average diameter 375.6 ± 5.9 nm. pattern consistent Ritger-Peppas model. also significantly improved condition. relative bioavailability found be 1.67-fold reference drug, indicating substantial increase broiler. Compared original enhanced, evidenced by reduction half MIC MBC values. These suggest improves making it promising candidate clinical use.